Police, along with Minto Fire and Guelph Wellington Paramedics responded to the scene just after 6 am.
The driver of the vehicle was pronounced deceased at the scene. The Office of the Chief Coroner was notified.
The highway was closed during the investigation by the West Region Traffic Incident Management and Enforcement team investigated the cause of the collision.
The road has reopened and reconstructionists continue to investigate.
Anyone with any information on the incident are asked to contact the Wellington County OPP at 1-888-310-1122.