Officers responded with members of the Walkerton Fire Department and Bruce Paramedics.
One person has died as a result of the collision.
The road is expected to be closed for a number of hours while the West Region OPP Traffic Incident Management and Enforcement Team attend and assist with the investigation.
More information will be provided as it becomes available.
Anyone with information is asked to call the South Bruce OPP at 1-888-310-1122. You can call anonymously to Crime Stoppers at 1-800-222-8477.