Officers were called to the scene Monday evening around 10:46pm on Perth Road 107.
The trailer was hauling 69 cows, two of which suffered severe burns and two died.
Approximately 35 were freed from the trailer.
The driver of the trailer was charged with causing damage or injury to animals being driven, obstruction of an officer, two counts of operating an unsafe vehicle, operating without insurance, failing to surrender CVOR, inspect or permit for vehicle and license.
Anyone with info on the incident is asked to contact the OPP or anonymously to Crime Stoppers.